PCR Equipment and Supplies
Invitrogen™ Platinum™ Direct PCR Universal Master Mix
Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.
Applied Biosystems™ Detection Enhancer, for real-time PCR
Formulated to improve template amplification in real-time PCR (qPCR) workflows
Invitrogen™ Oligo (dT) Primer (50 μM)
These are the same Invitrogen primers that are currently included in the SuperScript IV Kit (Cat. No. 18091050).
Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)
Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.
Applied Biosystems™ Absolute Q™ 1-step RT-dPCR Master Mix (4X)
Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X) is optimized for use with the QuantStudio Absolute Q Digital PCR System in a simple workflow with minimal processing steps.
Applied Biosystems™ GeneAmp™ dNTP Blend (2.5 mM ea)
GeneAmp dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH. Each vial consists of 1 mL of 2.5 mM each of dATP, dCTP, dGTP, and dTTP, yielding a 10 mM blend of dNTPs.
Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents
Applied Biosystems™ GeneAmp™ dNTP Blend (100 mM)
GeneAmp dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH. Each vial contains a blend of 100 mM solution of dNTP (25 mM each of dATP, dCTP, dGTP and dTTP).
Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.
Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
The TaqMan™ Cells-to-CT™ Express Kit enables rapid, reproducible gene expression analysis directly from cultured cells—allowing you to skip RNA extraction entirely. By removing extraction steps, the workflow reduces sample loss, shortens time to results, and improves consistency across experiments and operators.
Thermo Scientific™ dCTP Solution (100 mM)
Thermo Scientific dCTP (2'-deoxycytidine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase
Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.
Thermo Scientific™ dsDNase
dsDNase is a heat inactivatable recombinant shrimp DNase used to remove genomic DNA contamination of RNA samples before reverse transcription.
Invitrogen™ Template Switching Reverse Transcription Oligonucleotides
Use this set of oligonucleotides for template switching cDNA synthesis and preamplification. Template Switching Oligonucleotide and Capturing Oligo (dT) are used to incorporate adaptor sequences to full-length cDNA. Preamplification primer enables PCR amplification of obtained cDNA.
Thermo Scientific™ Phire Hot Start II DNA Polymerase
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.